EVALUATION OF THE POTENTIAL OF SAPONINS AND TANNINS OF DIALIUM GUINEENSE STEM BARK IN THE AMELIORATION OF CARBON TETRACHLORIDE-INDUCED RENAL OXIDATIVE STRESS
The potential of saponins and tannins extracted from Dialium guineense stem bark in ameliorating carbon tetrachloride (CCl4)-induced renal oxidative stress was examined. Adult male Wistar rats (n = 20) weighing 160 – 180 g (mean weight = 170 ± 10 g) were allocated randomly to four groups (5 rats per group). The rats received 150 mg/kg body weight bwt of the extracts orally for 28 days. Indices of oxidative stress were measured in rat kidneys. No significant changes existed between the concentrations of total protein among the groups (p > 0.05). The activities of all the antioxidant enzymes measured and reduced glutathione (GSH) levels were significantly lower in the CCl4 control group than in the normal control group, but they were increased by extract treatment. However, the level of malondialdehyde (MDA) elevated by CCl4 intoxication reduced after treatment (p > 0.05). These findings indicate that total saponins and tannins isolated from Dialium guineense stem bark could potentiate rat antioxidant defence in ameliorating CCl4-induced oxidative stress.