PRELIMINARY ANTIBIOGRAMIC STUDIES AND PHENOTYPIC DETECTION OF COLISTIN-RESISTANT PSEUDOMONAS SPECIES ISOLATED FROM POULTRY BIRDS IN BENIN CITY
Antibiotic-resistant bacteria have emerged as a serious threat to public health and a major cause of morbidity and mortality worldwide. The current study was aimed at investigating the prevalence of colistin-resistant Pseudomonas species from some selected poultry farms within Benin City, Edo State, Nigeria. A total of nineteen (19) fresh poultry droppings were collected aseptically from three (3) different poultry farms using a cold pack and taken to the Benson Idahosa University Laboratory for immediate analysis. Total heterotrophic bacterial and Pseudomonas counts were done by spread plate method using Nutrient Agar and Cetrimide Agar Base respectively. The antimicrobial susceptibility profiles of the Pseudomonas spp. were carried out according to the Kirby-Bauer disc diffusion method. Phenotypic resistance of the isolates to colistin was determined using the colistin antibiotics disc. The mean heterotrophic counts ranged from 29.67±7.09cfu/g to 240.33±53.26cfu/g, while the mean Pseudomonas counts were generally low, and ranged from 0.00±0.00cfu/g to 1.00±1.73cfu/g. All presumptive Pseudomonas species were resistant to ceftazidime, cefuroxime, augmentin, cefixime and nitrofurantion, as well as sensitivity to ciprofloxacin. Although, majority of the isolates were observed to be resistant to sparfloxacin (93.55%) and sensitive to ciprofloxacin (94.29%). The Multidrug resistant index recorded was between 41.18% and 94.12%. Out of the thirty-five (35) isolates screened for colistin-resistance, twenty-three (23) were positive. This study therefore implies that colistin-resistant Pseudomonas species are prevalent in poultry droppings.