IN VITRO ANTIBACTERIAL ACTIVITY OF LEAF EXTRACT FROM CHRYSOPHYLLUM ALBIDUM ON SOME BACTERIAL ISOLATES
Chrysophyllum albidum G. is a tropical plant and commonly found in Nigeria. It belongs to the sapotaceae family and used in folklore for the treatment of yellow fever, malaria, diarrhoea, vaginal and dermatological infections. The study was aimed at investigating the phytochemical composition and antibacterial
activity of methanol: sterile distilled water crude extracts from the leaves of Chrysophyllum albidum in the ratio 3:2 on some bacterial isolates. The phytochemical analysis of the crude extract of Chrysophyllum albidum revealed the presence of alkaloids, flavonoids, steroids, saponins, Cardiac glycosides and phlobalamins. The antimicrobial activity of the crude extract at a concentration of 30mg/ml, 35mg/ml and 40mg/ml was determined against some bacterial isolates using agar-diffusion method. The extract showed apparent antimicrobial activity in all concentrations against all bacterial isolates tested except Klebsiella pneumoniae, Methicillin resistant Staphylococcus aureus and Escherichia coli (Ek 58). The zone of inhibition exhibited by the extract ranged from 8 mm to 18 mm, 10 mm to 20 mm and 10 mm to 20 mm for concentrations of 30 mg/ml, 35 mg/ml and 40 mg/ml respectively. Streptomycin, which was used as a control also inhibited the growth of the bacterial isolates except Bacillus stearothermophillus (NCIB 822), Bacillus polymyxa (LIO), Pseudomonas flourescens (NCIB 3756). The
minimum inhibitory concentration (MIC) exhibited by the crude extract of Chrysophyllum albidum against the bacterial isolates ranged from 1.093mg/ml to 35mg/ml. The minimum bactericidal concentration of the crude extract ranged from 8.75 mg/ml to 35 mg/ml. The result obtained from this study revealed the importance of Chrysophyllum albidum which has a good potential as an antibacterial agent and further research is therefore suggested to isolate, characterize and elucidate bioactive compounds from C. albidum.