ANTIBACTERIAL ACTIVITIES, PHYTOCHEMICAL SCREENING AND CHROMATOGRAPHIC STUDIES OF MITRACARPUS SCABER (GIRDLEPOD) LEAF EXTRACTS
Plants have been used as valuable sources of natural products for maintaining animal and human health. The study investigated the phytochemical and antibacterial properties of the leaf extract of Mitracarpus scaber. The antibacterial effect of Mitracarpus scaber extracts was done using Agar well diffusion method with Mueller Hinton agar. Phytochemical composition of aqueous and ethanol extracts of Mitracarpus scaber leaves were determined using Gas chromatography-mass spectrometry. The study revealed that the concentration of phenols and tannins were more compared to other phytochemicals in both extracts when determined quantitatively. The observed phenolic content was 19.34±1.04 mg/100 g and 44.48±0.96 mg/100 g for the aqueous and ethanol
extracts respectively. While tannin content was 14.32±1.04 mg/100 g and 32.94±1.28 mg/100 g for the aqueous and ethanol extracts respectively. Both extracts inhibited the growth of Escherichia coli (15±2.00 mm and 23±1.00 mm), Klebsiella oxytoca (17±1.00 mm and 20 ± 2.00 mm), Pseudomonas aeruginosa (13±2.50 mm and 17±1.00 mm), Proteus mirabilis (16±1.00 mm and 22±1.00 mm), Enterococcus faecalis (18±1.00 mm and 24±1.00 mm) and Staphylococcus aureus (14±2.00 mm and 21±2.00 mm) at 100 mg/ml. Gas Chromatography-Mass Spectrometry confirmed the presence of 5-Nonanol (7.11%), 5-hydroxy-2-(hydroxyl methyl)- 4H-pyran-4-one (6.17%), 2-octenoic acid (16.70%), 1-hydroxy-2,2,6,6-tetramethyl-3- (4-nitroso-1-(piperazinylmethyl)- piperidin- 4-one (8.03%), Tetradecanoic acid (3.72%), Pentadecanoic acid (4.27%), 1, 2-Epoxyhexadecane (10.73%), Phytol (1.61%) and 11-bromoundecanoic acid (32.91%) in the aqueous extract. While the ethanol extract contained 5- Nonanol (29.71%), 5-hydroxy-2-(hydroxyl methyl)- 4H-pyran-4-one (24.95%), 2-octenoic acid (0.34%), 1- hydroxy-2,2,6,6-tetramethyl-3- (4-nitroso-1-(piperazinylmethyl)- piperidin-4-one (12.72%), Pentadecanoic acid (6.37%), 1, 2-epoxyhexadecane (0.07%), Phytol (2.53%), 6-octadecenoic acid (7.23%), 1,2-Benzenedicarboxylic acid (8.60%) and 11-bromoundecanoic acid (1.10%). The results of this study revealed that Mitracarpus scaber
leaves possesses antimicrobial effects.