ANTHOCYANIN EXTRACTED FROM WALNUT (JUGLAN REGIA) AND SOYBEAN (GLYCINE MAX) AS ANTI-BIOFILM AGENT AGAINST SPECIES OF PSEUDOMONAS AND KLEBSIELLA.

Abstract

Anthocyanins are phytochemicals whose extracts contained active principles that are antibiofilm that could potentially be administered to alleviate the risk of biofilm related-diseases and considered as a factor contributing to drug resistance in micro-organisms. The aim of this study was to determine the activity of
anthocyanin extract as natural effective alternate antibiofilm agent. The pulp of the fruits was extracted using 75% methanol, prior to sub-fractionation and lyophilization to obtain the anthocyanin component. The antibiofilm activity was performed using micro-dilution assay on iron and polystyrene (hydrocarbon) surfaces. Microbial growth was determined through optical density (OD650) while adhesion and biofilm formation was conducted using standard crystal violet staining methods. The results revealed that, both extracts of walnut and soybean reduced the microorganisms (Pseudomonas and Klebiella species) surveyed but the extract from soybean (Glycine max) was more effective (0.10 - 0475 mg/ml). In addition, increased extract concentration resulted in less biofilm
formation and demonstrated a considerable impact as natural inhibiting agent against the preliminary stages of biofilm formation with the capacity to disrupt the attachment to surfaces and with Klebsiella species, shown as the more susceptible organism.