INFLUENCE OF NITROGEN SOURCES ON THE PRODUCTION OF SINGLE CELL PROTEIN AND AMYLASE BY ASPERGILLUS NIGER USING WHEAT BRAN
The utilization of cheap available substrates such as wheat bran – an agro-waste, by microbes as feeds will enhance sustainable development. Therefore, the effect of nitrogen sources on the suitability of wheat bran medium for Single Cell Protein (SCP) and amylase production by Aspergillus niger was investigated. The nitrogen sources employed were Ammonium nitrate (NH4NO3), Ammonium chloride (NH4Cl), Sodium nitrate (NaNO3), Potassium nitrate (KNO3) and Ammonium sulphate ((NH4)2SO4). Biomass of A. niger, protein content, pH, amylase and reducing sugars were determined using standard methods in the course of submerged fermentation. The analysis showed that Ammonium sulphate medium produced the highest A. niger biomass yield, protein content and amylase activity of 9.50 ± 0.02 g/L, 5.20 ± 0.12 g/L and 326.12 ± 3.68 U/L respectively. However, the least protein content (3.50 ± 0.02 g/L) and amylase activity (256.01 ± 5.18 U/L) were from Sodium nitrate medium. Statistically, protein content from Ammonium sulphate medium was significantly different from other
nitrogen sources (p< 0.05) on day 6 of fermentation. This study demonstrated that A. niger biomass, SCP and
amylase production can be enhanced by supplementing wheat bran medium with ammonium sulphate.