CHARACTERIZATION AND IDENTIFICATION OF BACTERIAL ISOLATES FROM DIFFERENT DRINKING WATER SOURCES IN UROMI, EDO STATE, NIGERIA

  • E. O. Olatunji Biological Sciences Department, Samuel Adegboyega University, Ogwa,Edo State,Nigeria.
  • O. B. Isola Chemical Sciences Department, Samuel Adegboyega University, Ogwa,EdoState, Nigeria.
  • D. O. Fakeye Biological Sciences Department, Samuel Adegboyega University, Ogwa,Edo State,Nigeria.

Abstract

Samples of water which usually serve for drinking purposes from different sources inclusive of - river water, borehole, underground reservoir and sachet water in Uromi town, a semi urban area, were collected and analysed. The samples were investigated for colour, particle, odour, taste, pH, temperature, and bacteriological characteristics. Antibiotics susceptibility test was also carried out. The results showed no coliform in satchet water unlike the river, borehole and underground reservoir water. A total of sixteen bacterial isolates were isolated from the water samples namely: Corynebacterium sp, Escherichia coli, Pseudmonas sp., Salmonella sp., Klebsiella sp., Listeria sp., Staphylococcus aureus, Proteus sp., Serratia sp., Streptococcus sp., Vibrio sp., Flavobacterium sp.,
Bacillus sp., Bacillus sp., Micrococcus sp. and Corynebacterium sp. The river water had the highest heterotrophic count of 3.9 x 101 cfu/ml. The river water had the highest total coliform count. These results showed that the satchet water conform with NAFDAC and WHO standards for drinking water while the presence of coliform bacteria in the river, borehole and underground reservoir water showed they were contaminated and unsafe for direct drinking, thereby posing a health risk to the community dwellers if not properly treated. The percentage occurrence of the bacteria isolates showed that Staphylococcus sp., and Proteus sp had the highest percentage of occurrence, 12.46% while Flavobacterium sp., had the least, 1.78%. The gram positive isolates were susceptible to Ciprofloxacin, Norfloxacin, Centamycin, Streptomycin and Levofloxacin; they were all resistant to Rifampicine with the exception of Staphylococcus sp. The gramnegative isolates were susceptible to Ciprofloxacin and Centamycin. Preventive measures such as the proper treatment of drinking water should be encouraged

Author Biographies

E. O. Olatunji, Biological Sciences Department, Samuel Adegboyega University, Ogwa,Edo State,Nigeria.

Biological Sciences Department, Samuel Adegboyega University, Ogwa,Edo State,Nigeria.

O. B. Isola, Chemical Sciences Department, Samuel Adegboyega University, Ogwa,EdoState, Nigeria.

Chemical Sciences Department, Samuel Adegboyega University, Ogwa,EdoState, Nigeria.

D. O. Fakeye, Biological Sciences Department, Samuel Adegboyega University, Ogwa,Edo State,Nigeria.

Biological Sciences Department, Samuel Adegboyega University, Ogwa,Edo State,Nigeria.

Published
2019-07-03
How to Cite
OLATUNJI, E. O.; ISOLA, O. B.; FAKEYE, D. O.. CHARACTERIZATION AND IDENTIFICATION OF BACTERIAL ISOLATES FROM DIFFERENT DRINKING WATER SOURCES IN UROMI, EDO STATE, NIGERIA. SAU Science-Tech Journal, [S.l.], v. 4, n. 1, p. 37-52, july 2019. ISSN 2659-1529. Available at: <https://journals.sau.edu.ng/index.php/sjbas/article/view/125>. Date accessed: 05 june 2020.
Section
Articles