ANTIBACTERIAL AND ANTIOXIDANT ACTIVITIES OF CULTIVATED GANODERMA LUCIDUM (CURT.) P. KARST. IN NIGER-DELTA NIGERIA
Fruiting bodies of cultivated Ganoderma lucidum isolates were used in this study as against wild Ganoderma mushrooms to ascertain the viability of the cultivated specie. Crude extracts of Hot Water, Methanol, Ethanol, and Dichloromethane (20.0mg/ml) were prepared using mushroom powder of harvested fruit bodies. The antibacterial activity of various solvent extracts (20.0mg/ml) of Ganoderma lucidum was tested against five species of bacteria: Staphylococcus aureus, Streptococcus pneumoniae, Eschericia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae. Agar diffusion assay showed considerable activity against gram negative bacilli. Minimum inhibitory concentration values of the extracts showed that Dichloromethane extract exhibited maximum antibacterial activity (20.00 ± 0.29) in Eschericia coli, while the most susceptible bacterium observed was Klebsiella pneumoniae ( 20.67 ± 0.33) in Methanol extract. Extracts of harvested Ganoderma sp. exhibited strong antioxidant activity, the highest 1,1-Diphenly-2-picrylhydrazyl (DPPH) scavenging activity was in Dichloromethane extract (81.15%) in the various solvent extracts concentration of 20.0mg/ml. Due to dearth of information on indigenous Ganoderma lucidum, the positive results of screening of locally cultivated Ganoderma lucidum fruit bodies for antibacterial and antioxidant activities paves the platform for further phytochemical
studies and development of new resistant antibiotics.