EFFECT OF SELECTED NITROGEN SOURCES ON THE VEGETATIVE GROWTH OF DALDINIA CONCENTRICA (BOLTON) CES. & DE NOT.
This study was done in order to determine the effect of the Nitrogen sources on the vegetative growth of the mushroom, Daldinia concentrica. The Nitrogen sources selected were ammonium nitrate, calcium nitrate, peptone, urea, sodium nitrate and yeast extract. Among the nitrogen sources, only urea showed a significant (p=0.05) difference in the growth of the test mushroom. The mean diameter 5 days after inoculation was 1.94±0.80cm compared to peptone which had a mean diameter of 4.92±1.26cm. The other nitrogen sources such as ammonium nitrate, calcium nitrate and yeast extract statistically (p=0.05) compared with peptone and control. The mycelia density rating generally ranged from 3+ in urea to 5+ in some of the nitrogen sources much as ammonium nitrate and yeast extract. The cell length of the hyphae had the highest value (110.00 ±4.18 ?m) in sodium nitrate (NaNO3) while peptone gave the lowest value (68.00 ±2.55 ?m) compared to control with a mean length of 79.40 ±2.96 ?m. Only calcium nitrate and yeast extract gave the cell width value (18.20 ±0.92 and 18.20 ±0.98 ?m respectively) higher than the control (17.20 ±0.98 ?m) while the remaining nitrogen sources gave values significantly lower than control. The sources of Nitrogen was found to significantly (p=0.05) affect the mycelia biomass of D. concentrica. Among the nitrogen sources, Ammonium nitrate had the highest biomass (0.35 ±0.01g) while the lowest value (0.22 ±0.02g) was found on peptone.